Journal: The Journal of Clinical Investigation

Article Title: Monocytes and interstitial macrophages contribute to hypoxic pulmonary hypertension

doi: 10.1172/JCI176865

Figure Lengend Snippet: ( A ) Schematic showing hypoxia exposure time course in WT mice. Duration of hypoxia exposure is directly proportional to ( B ) RVSP and RV hypertrophy as measured by Fulton Index ( n = 6–13/group). At 3 days of hypoxia, increased protein expression of classical monocyte ligands ( C ) CCL2 ( n = 6–11/group) and ( D ) CCL12 ( n = 6–11/group), whereas significantly lower levels of nonclassical monocyte ligand ( E ) CX3CL1 ( n = 6/group) in the lungs. ( F ) Higher CCL2 gradient in lungs and in the ( G ) peripheral blood of WT mice following 3 days of hypoxia exposure ( n = 5/group) . Data in all panels were obtained from female mice. Statistical analysis was conducted using ANOVA, followed by Tukey’s post hoc test. * P < 0.05, ** P < 0.01, **** P < 0.0001. n = number of animals, mean ± SD; CI, confidence interval.

Article Snippet: Commercial ELISA kits were used for the quantification of CCL2 (Cat.#MJE00B, R&D Systems), CCL7 (Cat.# MBS764604, MyBioSource), CCL12 (Cat.#MCC120, R&D Systems), CXCL1 (Cat.#MCX310, R&D Systems), TSP-1 (Cat.# MBS264008, MyBioSource), and TGF-β1 (Cat.#sMB100B and DB100C, R&D Systems) using whole lung lysates and plasma samples from unexposed and hypoxia-exposed mice.

Techniques: Expressing

Journal: The Journal of Clinical Investigation

Article Title: Monocytes and interstitial macrophages contribute to hypoxic pulmonary hypertension

doi: 10.1172/JCI176865

Figure Lengend Snippet: ( A ) Schematic showing the experimental design and the dosing of DEX prophylaxis. Hypoxia-exposed WT mice treated with DEX prophylaxis had lower ( B ) RVSP and RVH by RHC ( n = 13–16/group). The blood ( C ) TSP-1 ( n = 5–11/group) and ( D ) TGF-β1 ( n = 5–9/group) levels were blunted by DEX prophylaxis treatment in hypoxic PH. The blood monocytes ligands ( E ) CCL2 ( n = 11/group) was significantly upregulated whereas ( F ) CCL12 ( n = 11/group) was downregulated following hypoxia exposure in the DEX prophylactically treated group. The levels of inflammatory proteins ( G ) TSP-1 ( n = 11/group) and ( H ) TGF-β1 ( n = 5–9/group) were significantly lower in the lung WT mice treated with DEX prophylaxis. DEX prophylaxis blunted classical monocyte ligands ( I ) CCL2 ( n = 11/group) and ( J ) CCL12 ( n = 11/group) in lung tissue lysates by ELISA. Data in all panels were obtained from female mice and followed a normal distribution. Statistical analysis was conducted using ANOVA, followed by Tukey’s post hoc test. mean ± SD plotted. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. RVSP, right ventricular systolic pressure; RV, right ventricle; IMs, interstitial macrophages; DEX, Dexamethasone.

Article Snippet: Commercial ELISA kits were used for the quantification of CCL2 (Cat.#MJE00B, R&D Systems), CCL7 (Cat.# MBS764604, MyBioSource), CCL12 (Cat.#MCC120, R&D Systems), CXCL1 (Cat.#MCX310, R&D Systems), TSP-1 (Cat.# MBS264008, MyBioSource), and TGF-β1 (Cat.#sMB100B and DB100C, R&D Systems) using whole lung lysates and plasma samples from unexposed and hypoxia-exposed mice.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of Neuroinflammation

Article Title: In vivo inhibition of miR-155 significantly alters post-stroke inflammatory response

doi: 10.1186/s12974-016-0753-x

Figure Lengend Snippet: Cytokine expression changes at 7 days after dMCAO. a Cytokine gene profiling at 7 days after dMCAO, inhibitor versus control groups. Clustergram of 84 common cytokines grouped by sample type (i1, i2, i3—samples collected from three different mice from the inhibitor group. c1, c2, c3—three samples from control group). Non-supervised hierarchical clustering was used to display common cytokine gene expression by heat map visualization, with dendrograms indicating coregulated genes across groups or individual samples. Results for two statistically different (between inhibitor and controls) genes Ccl12 and Cxcl3 are enlarged and showed in the bottom left corner . A color bar ( bottom ) relates color code to the magnitude of the differences in gene expression relative to the all-sample means for each gene. The results are also demonstrated in the form of scatter plots ( b ) of 84 common cytokine assays, revealing four upregulated and two downregulated genes (with more than twofold difference in expression between the inhibitor and control groups). Orange symbols identify upregulated genes, and blue symbols identify downregulated genes. c Protein expression levels of CCL12 and CXCL3 cytokines were assessed in the lesioned hemispheres of the animals from control ( red plots ) and inhibitor ( blue plots ) groups at 7,14, and 21 days after dMCAO, using specialized Mouse CCL12 and CXCL3 PicoKine™ ELISA kits (Boster Biological Technology). Cytokine expressions were also measured in the lesioned hemisphere of dMCAO-subjected mice at 48 h post-dMCAO ( orange diamond ). N = 6 mice per group/per time point. Two-way ANOVA followed by Tukey’s multiple comparison test; * p < 0.05. Error bars : SEM

Article Snippet: Orange symbols identify upregulated genes, and blue symbols identify downregulated genes. c Protein expression levels of CCL12 and CXCL3 cytokines were assessed in the lesioned hemispheres of the animals from control ( red plots ) and inhibitor ( blue plots ) groups at 7,14, and 21 days after dMCAO, using specialized Mouse CCL12 and CXCL3 PicoKineTM ELISA kits (Boster Biological Technology).

Techniques: Expressing, Control, Gene Expression, Enzyme-linked Immunosorbent Assay, Comparison